EBI3 Protein

Catalog No. ABIN6700896

Supplier Product No.: 009-001-b66

Recombinant EBI3 protein expressed in Escherichia coli (E. coli).

Quick Overview for EBI3 Protein (ABIN6700896)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 90,00%

Product Details

Supplier Product No.: 009-001-b66

Supplier: Rockland

Purpose: EBI-3 Human Recombinant Protein

Purification: Recombinant protein corresponds to amino acids 21 to 229 of mature human EBI3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Assay data for Human recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Application Details

Application Notes:

Application Note: EBI-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays. The product is lyophilized from a sterile filtered aqueous solution of 0.1 % TFA and 0.5 % mannitol.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: 0.5 % Mannitol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: IL-27B

Background:

Synonyms: Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B cytokine, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35

Background: Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Human EBI is a non-glycosylated polypeptide chain consisting of 209 amino acids with a molecular weight of 23.3 kDa.

UniProt: Q14213