EBI3 Protein

Catalog No. ABIN6700897

Supplier Product No.: 010-001-b66

This Recombinant EBI3 protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for EBI3 Protein (ABIN6700897)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 90,00%

Product Details

Supplier Product No.: 010-001-b66

Supplier: Rockland

Purpose: EBI-3 Mouse Recombinant Protein

Purification: Recombinant protein corresponding to amino acids 2 to 207 of mature mouse EBI-3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC, each against a known standard.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Application Details

Application Notes:

Application Note: EBI-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

References

Nikolic, Ruiz-Garrido, Crespo, Romero-Becerra, Leiva-Vega, Mora, León, Rodríguez, Leiva, Plata-Gómez, Alvarez Flores, Torres, Hernández-Cosido, López, Vázquez, Efeyan, Martin, Marcos, Sabio: "Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis." in: EMBO reports, Vol. 25, Issue 6, pp. 2635-2661, (2024) (PubMed).

Target Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: Ebi3

Background:

Synonyms: Interleukin-27 subunit beta precursor [Mus musculus], mouse EBI-3 cytokine, EBI3, EBI-3, IL35, IL-35

Background: Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a molecular weight of 22,900 Da.

Gene ID: 50498

UniProt: O35228