VHL Protein (AA 1-172) (GST tag)
(1 reference)Quick Overview for VHL Protein (AA 1-172) (GST tag) (ABIN1325000)
Target
See all VHL ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-172
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Purification tag / Conjugate
- This VHL protein is labelled with GST tag.
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Purpose
- VHL (Human) Recombinant Protein (P01)
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Sequence
- MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD
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Characteristics
- Human VHL full-length ORF ( ENSP00000344757, 1 a.a. - 172 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-213) protein (His tag)
custom-made VHL Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-154) protein (His tag)VHL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)VHL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "WSB1 promotes tumor metastasis by inducing pVHL degradation." in: Genes & development, Vol. 29, Issue 21, pp. 2244-57, (2015) (PubMed).
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: "WSB1 promotes tumor metastasis by inducing pVHL degradation." in: Genes & development, Vol. 29, Issue 21, pp. 2244-57, (2015) (PubMed).
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- VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))
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Alternative Name
- VHL
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Background
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Full Gene Name: von Hippel-Lindau tumor suppressor
Synonyms: HRCA1,RCA1,VHL1 -
Gene ID
- 7428
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Pathways
- Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway
Target
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