Human Adenovirus type 3 (HAdV-3) Protein

Catalog No. ABIN6941928

Recombinant Human Adenovirus type 3 protein expressed in HEK-293 Cells.

Quick Overview for Human Adenovirus type 3 (HAdV-3) Protein (ABIN6941928)

Target: Human Adenovirus type 3 (HAdV-3)

Protein Type: Recombinant

Origin: Adenovirus

Source: HEK-293 Cells

Purity: Purified virus

Product Details

Purpose: Wild type Adenovirus type 3. Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis. Safety category BSL2.

Characteristics: Adenovirus Type 3 Particles, Wild-type
Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.

Purification: Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.

Application Details

Application Notes: 2.53x10^12 particles per mL (BCA assay)

Comment:

This product is a concentrated source of highly-purified human Adenovirus type 3 particles (wild-type) from a lysate of optimally-infected 293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, this Ad3 preparation is of very high quality and minimal lot-to-lot variation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 50 mM Hepes pH 7.8, 10 % Glycerol

Storage: -80 °C

Storage Comment: -80°C

Target Details

Target: Human Adenovirus type 3 (HAdV-3)

Alternative Name: Adenovirus Type 3

Target Type: Virus

Background: Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).