CCL14 Protein (AA 28-93) (Biotin)

Catalog No. ABIN6952143

This Recombinant CCL14 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL14 Protein (AA 28-93) (Biotin) (ABIN6952143)

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Migration Assay (CMA)

Purity: > 97 % by SDS PAGE

Product Details

Protein Characteristics: AA 28-93

Purification tag / Conjugate: This CCL14 protein is labelled with Biotin.

Sequence: GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN

Characteristics: Biotininylated Recombinant Human CCL14 (HCC-1)

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 0.2-0.4 nM determined by migration assay with cells expressing recombinant CCR1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Storage: -20 °C,-80 °C

Storage Comment: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Alternative Name: CCL14 (HCC-1)

Background: Hemofiltrate CC chemokine-1(HCC-1/CCL14) is endogeneously expressed by numerous tissues. Upon processing of the N terminal residues of the full length HCC-1 by the uPA-plasmin system, the active form of HCC-1 (made by ChemoTactics) is a strong agonist for CCR1, CCR5 and to a lesser extent CCR3, and causes chemotaxis of different types of leukocytes. The active form of HCC-1 is also shown as a potent inhibitor of HIV entry.

Molecular Weight: 10.220 kDa

UniProt: Q16627