CCL27 Protein (AA 25-112)

Catalog No. ABIN6952170

This Recombinant CCL27 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL27 Protein (AA 25-112) (ABIN6952170)

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Migration Assay (CMA)

Purity: > 97 % by SDS PAGE

Product Details

Protein Characteristics: AA 25-112

Sequence: FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG

Characteristics: Recombinant Human CCL27 (CTACK)

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 34 nM determined by Migration Assay in cells expressing recombinant CCR10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Storage: -20 °C,-80 °C

Storage Comment: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Alternative Name: CCL27 (CTACK)

Background: Cutaneous T-cell-attracting chemokine (CTACK/CCL27) is a ligand for cell surface receptor CCR10. It is responsible for chemotaxis of skin-homing memory T cells during cutaneous inflammation. Interestingly, after CCR10-mediated internalization, CTACK, as well as a non-secreted splice variant of the same gene, can reach the nucleus and modulate transcription and cell behavior.

Molecular Weight: 10.149kDa

UniProt: Q9Y4X3