CCL3 Protein (AA 24-92) (Biotin)

Catalog No. ABIN6952184

Recombinant CCL3 protein expressed in Escherichia coli (E. coli).

Quick Overview for CCL3 Protein (AA 24-92) (Biotin) (ABIN6952184)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Migration Assay (CMA)

Purity: > 97 % by SDS PAGE

Product Details

Protein Characteristics: AA 24-92

Purification tag / Conjugate: This CCL3 protein is labelled with Biotin.

Sequence: SLAADTPTAC CFSYTSRQIP QNFIADYFET SSQCSKPGVI FLTKRSRQVC ADPSEEWVQK YVSDLELSA

Characteristics: Biotininylated Recombinant Human CCL3 (MIP-1alpha)

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 1.1-2.6 nM determined by Migration Assay of recombinant CCR5 containing cells

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Storage: -20 °C,-80 °C

Storage Comment: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: CCL3 (MIP-1alpha)

Background: Macrophage inflammatory proteins 1-alpha (MIP1alpha)(CCL3) binds to cell surface receptors CCR1 and CCR5. It is proinflammatory, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells. Moreover, by binding to one of the HIV coreceptors, CCR5, it suppresses HIV infection.

Molecular Weight: 10.135 kDa

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy