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CCL7 Protein (AA 24-99) (Biotin)

Recombinant CCL7 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN6952211

Quick Overview for CCL7 Protein (AA 24-99) (Biotin) (ABIN6952211)

Target

See all CCL7 Proteins
CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 10
  • 6
  • 5
Human

Source

  • 17
  • 2
  • 1
  • 1
Escherichia coli (E. coli)

Application

Cell Migration Assay (CMA)

Purity

> 97 % by SDS PAGE
  • Protein Characteristics

    AA 24-99

    Purification tag / Conjugate

    This CCL7 protein is labelled with Biotin.

    Sequence

    QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL

    Characteristics

    Biotininylated Recombinant Human CCL7 (MCP-3)

    Endotoxin Level

    <0.01 EU per 1μg of the protein by the LAL method

    Biological Activity Comment

    EC50 = 1.4-2.6 nM determined by migration assay with cells expressing recombinant CCR2
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

    Storage

    -20 °C,-80 °C

    Storage Comment

    12 months from date of receipt, -20 to -70 °C as supplied.
    Suggest to use immediately after reconstitution
    1 month at -20 to -70 °C under sterile conditions after reconstitution.

    Expiry Date

    12 months
  • Target

    CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

    Alternative Name

    CCL7 (MCP-3)

    Background

    Monocyte chemotactic protein 3 (MCP-3/CCL7) is secreted by monocytes and certain tumor cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP-3 binds and signals through several chemokine receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5.

    Molecular Weight

    11.374 kDa

    UniProt

    P80098
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