CD40 Ligand Protein (CD40LG) (AA 116-261) (DYKDDDDK Tag,His tag)

Catalog No. ABIN6952262

Recombinant CD40 Ligand protein expressed in HEK-293 Cells.

Quick Overview for CD40 Ligand Protein (CD40LG) (AA 116-261) (DYKDDDDK Tag,His tag) (ABIN6952262)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 116-261

Purification tag / Conjugate: This CD40 Ligand protein is labelled with DYKDDDDK Tag,His tag.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand

Background: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.

Molecular Weight: 49.9 kDa

NCBI Accession: NP_000065

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints