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SARS-CoV-2 Spike S1 (RBD), (W436R) protein (His tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. ELISA, SDS
Pubmed (1)
Catalog No. ABIN6952632
$490.00
Plus shipping costs $45.00 and $24.00 dry ice
100 μg ABIN6952632
100 μg ABIN6952632
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  • Target
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Protein Characteristics
    RBD, W436R
    Origin
    • 70
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 61
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS)
    Purpose
    SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
    Sequence
    AA 319-541
    Characteristics
    SARS-CoV-2 S protein RBD (W436R), His Tag (SPD-S52H7) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (W436R)). Predicted N-terminus: Arg 319
    This protein carries a polyhistidine tag at the C-terminus.
    The W436R mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    4-8°C for 12 months in lyophilized state,
    -70°C for 3 years under sterile conditions after reconstitution.
  • Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." in: Nature communications, Vol. 12, Issue 1, pp. 2623, 2021 (PubMed).

  • Target
    SARS-CoV-2 Spike S1
    Synonyms
    E2, Surface Glycoprotein, S
    Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    27.0 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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