SARS-CoV-2 NSP3 Protein (His tag)

Catalog No. ABIN6952636

Product Details

Target: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SARS-CoV-2 NSP3 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purpose: SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

Sequence: AA 746-1060

Characteristics: SARS-CoV-2 Papain-like Protease, His Tag is expressed from E.coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt,
-70°C for 3 months under sterile conditions.

References

Kim, Seiler, Jones, Ridout, Camp, Fabrizio, Jeevan, Miller, Throm, Ferrara, Fredrickson, Lowe, Wang, Odemuyiwa, Wan, Webby: "Antibody Responses to SARS-CoV-2 Antigens in Humans and Animals." in: Vaccines, Vol. 8, Issue 4, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Abstract: NSP3 Products

Synonyms: pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein

Target Type: Viral Protein

Background: New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.

Molecular Weight: 37.6 kDa

NCBI Accession: YP_009725299