SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) protein (His tag)
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- Target See all SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) products
- SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Purpose
- SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag
- Sequence
- AA 1-198
- Characteristics
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SARS-CoV-2 NSP8, His Tag (NS8-C5149) is expressed from E.coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1).Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus. - Purity
- >95 % as determined by SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Tris, 250 mM NaCl, pH 8.5
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
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The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.
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- Target
- SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
- Alternative Name
- NSP8 (SARS-CoV NSP8 Products)
- Synonyms
- pp1a Protein, pp1ab Protein, orf1ab polyprotein (pp1ab) Protein, orf1ab Protein
- Target Type
- Viral Protein
- Background
- NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.
- Molecular Weight
- 23.9 kDa
- NCBI Accession
- YP_009725304
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