SARS-CoV-2 Spike S1 (RBD) protein (His-SUMOstar Tag) Protein
- SARS-CoV-2 Spike S1
- Protein Type
- Protein Characteristics
- SARS Coronavirus-2 (SARS-CoV-2)
- Purification tag / Conjugate
- This SARS-CoV-2 Spike S1 protein is labelled with His-SUMOstar Tag.
- RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF
- N-terminal 6xHis-sumostar-tagged
- Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin) Protein
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.SARS-CoV-2 Spike S1 (G476S), (RBD) protein (His tag) Protein
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE.
> 90 % as determined by SEC-MALS. ELISA, SDSSARS-CoV-2 Spike S1 (B.1.351 - beta), (RBD) (Active) protein (His tag) Protein
Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2, the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.
- For Research Use only
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C.
- Tris/PBS-based buffer, 6 % Trehalose, pH 8.0
- -20 °C
- Storage Comment
- Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- SARS-CoV-2 Spike S1
- E2, Surface Glycoprotein, S
- Target Type
- Viral Protein
- Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.
- Gene ID