Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SARS-CoV-2 Spike S1 (RBD) protein (His-SUMOstar Tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Yeast Recombinant Greater than 85 % as determined by SDS-PAGE.
Catalog No. ABIN6953166
$2,094.40
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 8 to 11 Business Days
  • Target
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Protein Characteristics
    RBD
    Origin
    • 70
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 62
    • 3
    • 2
    • 1
    • 1
    • 1
    Yeast
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His-SUMOstar Tag.
    Sequence
    RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF
    Characteristics
    N-terminal 6xHis-sumostar-tagged
    Purity
    Greater than 85 % as determined by SDS-PAGE.
  • Comment

    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2, the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C.
    Buffer
    Tris/PBS-based buffer, 6 % Trehalose, pH 8.0
    Storage
    -20 °C
    Storage Comment
    Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • Target
    SARS-CoV-2 Spike S1
    Synonyms
    E2, Surface Glycoprotein, S
    Target Type
    Viral Protein
    Background
    Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.
    Gene ID
    43740568
    UniProt
    P0DTC2
You are here:
help Support