Syndecan 1 Protein (SDC1) (AA 23-254) (His tag,Fc Tag)

Catalog No. ABIN6961079

Recombinant Syndecan 1 protein expressed in HEK-293 Cells.

Quick Overview for Syndecan 1 Protein (SDC1) (AA 23-254) (His tag,Fc Tag) (ABIN6961079)

Target: Syndecan 1 (SDC1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-254

Purification tag / Conjugate: This Syndecan 1 protein is labelled with His tag,Fc Tag.

Purpose: Recombinant human CD138 protein with C-terminal human Fc and 6xHis tag

Specificity: CD138(Gln23-Gly254) hFc(Glu99-Ala330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Syndecan 1 (SDC1)

Alternative Name: CD138

Background: SDC1,Syndecan-1,CD138,SYND1,SDC
Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

Molecular Weight: predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of CD138-hFc-His is 70-100 kDa due to glycosylation.

Gene ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism