TNFSF4 Protein (AA 99-330) (His tag,mFc Tag)

Catalog No. ABIN6961094

Recombinant TNFSF4 protein expressed in HEK-293 Cells.

Quick Overview for TNFSF4 Protein (AA 99-330) (His tag,mFc Tag) (ABIN6961094)

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This TNFSF4 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human OX40L protein with N-terminal mouse Fc and C-terminal 6xHis tag

Specificity: MFc(Pro99-Lys330) OX40 Ligand(Gln51-Leu183) 6xHis

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Alternative Name: OX40 Ligand

Background: OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

Molecular Weight: predicted molecular mass of 44.0 kDa after removal of the signal peptide.

Gene ID: 7292

UniProt: P23510

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints