P-Selectin Protein (AA 42-771) (Fc Tag)

Catalog No. ABIN6961139

Recombinant P-Selectin protein expressed in HEK-293 Cells.

Quick Overview for P-Selectin Protein (AA 42-771) (Fc Tag) (ABIN6961139)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 42-771

Purification tag / Conjugate: This P-Selectin protein is labelled with Fc Tag.

Purpose: Recombinant human SELP protein with C-terminal human Fc tag

Specificity: SELP(Trp42-Aal771) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: SELP

Background: P-Selectin,CD62P,SELP,GMP-140
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.

Molecular Weight: predicted molecular mass of 106.1 kDa after removal of the signal peptide.The apparent molecular mass of SELP-hFc is 130-150 kDa due to glycosylation.

Gene ID: 6403

UniProt: P16109