IgG1 Fc (AA 99-330) Protein

Catalog No. ABIN6961177

This Recombinant IgG1 Fc protein is expressed in HEK-293 Cells.

Quick Overview for IgG1 Fc (AA 99-330) Protein (ABIN6961177)

Target: IgG1 Fc

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purpose: Recombinant Human IgG1-Fc Protein

Specificity: HFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IgG1 Fc

Alternative Name: IgG1-Fc

Background: IgG1 Fc Protein
IGHG1 (Immunoglobulin Heavy Constant Gamma 1 (G1m Marker)) is a Protein Coding gene. Diseases associated with IGHG1 include Leukemia,Chronic Lymphocytic and Heavy Chain Deposition Disease. Among its related pathways are Interleukin-4 and 13 signaling and IL4-mediated signaling events. Gene Ontology (GO) annotations related to this gene include antigen binding. An important paralog of this gene is IGHG3.

Molecular Weight: predicted molecular mass of 27.7 kDa after removal of the signal peptide.The apparent molecular mass of IgG1-Fc is 30-40 kDa due to glycosylation.

Gene ID: 2214

UniProt: P01857