NG2 Protein (AA 30-2224) (His tag)

Catalog No. ABIN6964100

Recombinant NG2 protein expressed in HEK-293 Cells.

Quick Overview for NG2 Protein (AA 30-2224) (His tag) (ABIN6964100)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-2224

Purification tag / Conjugate: This NG2 protein is labelled with His tag.

Purpose: Recombinant Human CSPG4 Protein with C-terminal 6xHis tag.

Specificity: CSPG4(Ala30-Ser2224) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4

Background: CSPG4A,HMW-MAA,MCSP,MCSPG,MEL-CSPG,MSK16,NG2
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 237.7 kDa after removal of the signal peptide.

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process