ANGPTL3 Protein (AA 17-221) (His tag)

Catalog No. ABIN6964117

This Recombinant ANGPTL3 protein is expressed in HEK-293 Cells.

Quick Overview for ANGPTL3 Protein (AA 17-221) (His tag) (ABIN6964117)

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 17-221

Purification tag / Conjugate: This ANGPTL3 protein is labelled with His tag.

Purpose: Recombinant human ANGPTL3 protein with C-terminal 6xHis tag

Specificity: ANGPTL3(Ser17-Arg221) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Alternative Name: ANGPTL3

Background: ANG-5,ANGPT5,ANL3,FHBL2
This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.

Molecular Weight: predicted molecular mass of 24.8 kDa after removal of the signal peptide.The apparent molecular mass of ANGPTL3-His is 25-35 kDa due to glycosylation.

Gene ID: 27329

UniProt: Q9Y5C1