CD11b Protein (AA 17-1104) (Fc Tag)

Catalog No. ABIN6964128

This Recombinant CD11b protein is produced in HEK-293 Cells.

Quick Overview for CD11b Protein (AA 17-1104) (Fc Tag) (ABIN6964128)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 17-1104

Purification tag / Conjugate: This CD11b protein is labelled with Fc Tag.

Purpose: Recombinant human ITGAM Protein with C-terminal human Fc tag

Specificity: ITGAM(Phe17-Asn1104) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: affinity purification

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM

Background: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Molecular Weight: predicted molecular mass of 146.6 kDa after removal of the signal peptide.

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation