C5AR1 Protein (AA 1-37) (Fc Tag)

Catalog No. ABIN6964150

This Recombinant C5AR1 protein is expressed in HEK-293 Cells.

Quick Overview for C5AR1 Protein (AA 1-37) (Fc Tag) (ABIN6964150)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-37

Purification tag / Conjugate: This C5AR1 protein is labelled with Fc Tag.

Purpose: Recombinant Human C5AR1 Protein with C-terminal human Fc tag

Specificity: C5AR1(Met1-Asp37) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5AR1

Background: C5A,C5AR,C5R1,CD88
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520, PubMed:29300009). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:8182049, PubMed:7622471, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.

Molecular Weight: predicted molecular mass of 30.4 kDa after removal of the signal peptide.The apparent molecular mass of C5AR1-hFc is 40-53 kDa due to glycosylation.

UniProt: P21730

Pathways: Complement System