CD9 Protein (CD9) (AA 112-195) (His tag)

Catalog No. ABIN6964169

Recombinant CD9 protein expressed in HEK-293 Cells.

Quick Overview for CD9 Protein (CD9) (AA 112-195) (His tag) (ABIN6964169)

Target: CD9

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 112-195

Purification tag / Conjugate: This CD9 protein is labelled with His tag.

Purpose: Recombinant human CD9 protein with C-terminal 6xHis tag

Specificity: CD9(Ser112-Ile195) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD9

Alternative Name: CD9

Background: BTCC-1,DRAP-27,MIC3,MRP-1,TSPAN-29,TSPAN29
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Molecular Weight: predicted molecular mass of 10.5 kDa after removal of the signal peptide. The apparent molecular mass of CD9-His is 10-15 kDa due to glycosylation.

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization