ITGAV & ITGB1 (AA 31-992) protein (His tag,Fc Tag)

Catalog No. ABIN6964181

Recombinant ITGAV & ITGB1 protein expressed in HEK-293 Cells.

Quick Overview for ITGAV & ITGB1 (AA 31-992) protein (His tag,Fc Tag) (ABIN6964181)

Target: ITGAV & ITGB1

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 31-992

Purification tag / Conjugate: His tag,Fc Tag

Purpose: Recombinant heterodimer protein containg both human ITGAV Protein with C-terminal 6xHis tag and human ITGB1 protein with C-terminal human Fc tag

Specificity: ITGAV(Phe31-Val992) 6xHis tag - ITGB1(Gln21-Asp728) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ITGAV & ITGB1

Background: Integrin alpha V beta 1,ITGAVandITGB1
Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.

Molecular Weight: predicted molecular mass of 107.1 and 104.5 kDa after removal of the signal peptide. The apparent molecular mass of ITGAV-His and ITGB1-hFc is 130-250 kDa due to glycosylation.

UniProt: P06756