IL1RN Protein (AA 18-332) (Fc Tag)

Catalog No. ABIN6964205

Recombinant IL1RN protein expressed in HEK-293 Cells.

Quick Overview for IL1RN Protein (AA 18-332) (Fc Tag) (ABIN6964205)

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 18-332

Purification tag / Conjugate: This IL1RN protein is labelled with Fc Tag.

Purpose: Recombinant Human IL1RA Protein with C-terminal human Fc tag

Specificity: IL1RA(Leu18-Thr332) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RA

Background: CD121A,D2S1473,IL-1R-alpha,IL1R,IL1RA,P80
This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]

Molecular Weight: predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL1RA-hFc is 55-70 kDa due to glycosylation.

UniProt: P14778

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints