MAGEA4 Protein (AA 1-317) (His tag)

Catalog No. ABIN6964213

This Recombinant MAGEA4 protein is expressed in HEK-293 Cells.

Quick Overview for MAGEA4 Protein (AA 1-317) (His tag) (ABIN6964213)

Target: MAGEA4 (Melanoma Antigen Family A, 4 (MAGEA4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-317

Purification tag / Conjugate: This MAGEA4 protein is labelled with His tag.

Purpose: Recombinant human MAGEA4 protein with C-terminal 6xHis tag

Specificity: MAGEA4(Met1-Val317) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MAGEA4 (Melanoma Antigen Family A, 4 (MAGEA4))

Alternative Name: MAGEA4

Background: CT1.4
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80 % sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2020]

Molecular Weight: predicted molecular mass of 35.7 kDa after removal of the signal peptide. The apparent molecular mass of MAGEA4-His is 35-55 kDa due to glycosylation.

UniProt: P43358