K-RAS Protein (AA 2-185) (Fc Tag)

Catalog No. ABIN6964285

This Recombinant K-RAS protein is expressed in HEK-293 Cells.

Quick Overview for K-RAS Protein (AA 2-185) (Fc Tag) (ABIN6964285)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 2-185

Purification tag / Conjugate: This K-RAS protein is labelled with Fc Tag.

Purpose: Recombinant Human KRAS with C-terminal human Fc tag

Specificity: KRAS (Thr2-Cys185) hFc (Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Target Type: Viral Protein

Background: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 47.1 kDa after removal of the signal peptide. The apparent molecular mass of KRAS-hFc is 35-55 kDa due to glycosylation.

UniProt: P01116