K-RAS Protein (Gly12Val) (His tag)

Catalog No. ABIN7535081

Product Details

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Gly12Val

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This K-RAS protein is labelled with His tag.

Purpose: Active Recombinant Human GTPase KRas/KRAS(G12V) Protein

Sequence: TEYKLVVVGA VGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC LLDILDTAGQ EEYSAMRDQY MRTGEGFLCV FAINNTKSFE DIHHYREQIK RVKDSEDVPM VLVGNKCDLP SRTVDTKQAQ DLARSYGIPF IETSAKTRQR VEDAFYTLVR EIRQYRLKKI SKEEKTPGCV KIKKC

Specificity: Thr2-Cys186(G12V)

Purity: > 85 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human KRAS, His Tag at 2 μg/mL (100 μL/well) can bind Anti-KRAS Ab with a linear range of 42-84.56 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: GTPase KRas/KRAS (KRAS Products)

Synonyms: wu:fa04e08 Protein, wu:fc14b12 Protein, wu:fc23g10 Protein, wu:fj89d12 Protein, zgc:85725 Protein, Kras2 Protein, c-Ki-ras Protein, p21 Protein, C-K-RAS Protein, CFC2 Protein, K-RAS2A Protein, K-RAS2B Protein, K-RAS4A Protein, K-RAS4B Protein, KI-RAS Protein, KRAS1 Protein, KRAS2 Protein, NS Protein, NS3 Protein, RASK2 Protein, AI929937 Protein, K-ras Protein, Ki-ras Protein, Kras-2 Protein, p21B Protein, ras Protein, k-ras Protein, kras Protein, kras-a Protein, kras-b Protein, rask2 Protein, K-RAS Protein, p21ras Protein, KRAS proto-oncogene, GTPase Protein, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Protein, Kirsten rat sarcoma viral oncogene homolog Protein, semicolonial-7 Protein, Ras family protein Protein, GTPase KRas Protein, Kirsten rat sarcoma viral oncogene homolog S homeolog Protein, KRAS Protein, kras Protein, Kras Protein, smco-7 Protein, PGTG_03066 Protein, Tsp_02666 Protein, Tsp_01642 Protein, rask Protein, kras.S Protein

Target Type: Viral Protein

Background: KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, c-Ki-ras2, GTPase KRas,C-K-RAS,CFC2,K-RAS2A,K-RAS2B,K-RAS4A,K-RAS4B,KI-RAS,KRAS1,KRAS2,NS,NS3,RALD,RASK2,c-Ki-ras2

Gene ID: 3845

UniProt: P01116