XAGE1 Protein (AA 99-330) (Fc Tag)

Catalog No. ABIN6964346

Recombinant XAGE1 protein expressed in HEK-293 Cells.

Quick Overview for XAGE1 Protein (AA 99-330) (Fc Tag) (ABIN6964346)

Target: XAGE1 (XAGE1A) (X Antigen Family, Member 1A (XAGE1A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This XAGE1 protein is labelled with Fc Tag.

Purpose: Recombinant Human XAGE1A Protein with N-terminal human Fc tag

Specificity: HFc(Glu99-Ala330) XAGE1A(Met1-Val81)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: XAGE1 (XAGE1A) (X Antigen Family, Member 1A (XAGE1A))

Alternative Name: XAGE1A

Background: CT12.1,CT12.1A,CTP9,GAGED2,XAGE1
This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]

Molecular Weight: predicted molecular mass of 35.2 kDa after removal of the signal peptide. The apparent molecular mass of hFc-XAGE1A is 35-55 kDa due to glycosylation.

UniProt: Q9HD64