alpha Fetoprotein Protein (AA 19-609) (Fc Tag)

Catalog No. ABIN7092671

This Recombinant alpha Fetoprotein protein is expressed in HEK-293 Cells.

Quick Overview for alpha Fetoprotein Protein (AA 19-609) (Fc Tag) (ABIN7092671)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-609

Purification tag / Conjugate: This alpha Fetoprotein protein is labelled with Fc Tag.

Purpose: Recombinant human AFP protein with C-terminal human Fc tag

Specificity: AFP(Arg19-Val609) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background: AFPD,FETA,HPAFP
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Molecular Weight: predicted molecular mass of 92.6 kDa after removal of the signal peptide.The apparent molecular mass of AFP-hFc is 100-130 kDa due to glycosylation.

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process