DKK1 Protein (AA 32-266) (Fc Tag)

Catalog No. ABIN7092683

This Recombinant DKK1 protein is expressed in HEK-293 Cells.

Quick Overview for DKK1 Protein (AA 32-266) (Fc Tag) (ABIN7092683)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 32-266

Purification tag / Conjugate: This DKK1 protein is labelled with Fc Tag.

Purpose: Recombinant human DKK1 Protein with C-terminal human Fc tag

Specificity: DKK1(Thr32-His266) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: DKK-1,SK
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.

Molecular Weight: predicted molecular mass of 51.9 kDa after removal of the signal peptide.The apparent molecular mass of DKK1-hFC is 70 kDa due to glycosylation.

Gene ID: 22943

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation