APP Protein (AA 18-669) (His tag)

Catalog No. ABIN7092740

This Recombinant APP protein is expressed in HEK-293 Cells.

Quick Overview for APP Protein (AA 18-669) (His tag) (ABIN7092740)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 18-669

Purification tag / Conjugate: This APP protein is labelled with His tag.

Purpose: Recombinant Human APP Protein with C-terminal 6xHis tag

Specificity: APP(Leu18-Val669) 6xHis Tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background: AAA,ABETA,ABPP,AD1,APPI,CTFgamma,CVAP,PN-II,PN2,preA4
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Molecular Weight: predicted molecular mass of 74.6 kDa after removal of the signal peptide.The apparent molecular mass of APP-His is 100-130 kDa due to glycosylation.

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour