EVA1C Protein (AA 49-320) (Fc Tag)

Catalog No. ABIN7092769

This Recombinant EVA1C protein is produced in HEK-293 Cells.

Quick Overview for EVA1C Protein (AA 49-320) (Fc Tag) (ABIN7092769)

Target: EVA1C (Eva-1 Homolog C (EVA1C))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 49-320

Purification tag / Conjugate: This EVA1C protein is labelled with Fc Tag.

Purpose: Recombinant Human EVA1C with C-terminal human Fc tag

Specificity: EVA1C(Leu49-Glu320) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: EVA1C (Eva-1 Homolog C (EVA1C))

Alternative Name: EVA1C

Background: B18,B19,C21orf63,C21orf64,FAM176C,PRED34,SUE21
EVA1C (aliases C21orf63), first identified in 2001, is a membrane protein encoding-gene. EVA1C protein has been found in a variety of human tissues. Kanae Mitsunaga identified EVA1C protein possessing two repeats of putative 'galactose-binding lectin domains' that bind heparin. Although the role of EVA1C has not been reported in tumor, Manas Kotepui et al. reported that ADGRL3 (LPHN3), an important paralog of EVA1C gene, was upregulated in breast cancer and was correlated with axillary lymph node metastasis.

Molecular Weight: predicted molecular mass of 56.6 kDa after removal of the signal peptide. The apparent molecular mass of EVA1C-hFc is 55-70 kDa due to glycosylation.

UniProt: P58658