F4/80 Protein (AA 21-599) (His tag)

Catalog No. ABIN7092778

This Recombinant F4/80 protein is produced in HEK-293 Cells.

Quick Overview for F4/80 Protein (AA 21-599) (His tag) (ABIN7092778)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-599

Purification tag / Conjugate: This F4/80 protein is labelled with His tag.

Purpose: Recombinant human ADGRE1 protein with C-terminal 6xHis tag

Specificity: ADGRE1(His21-Asp599) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: ADGRE1

Background: EMR1,TM7LN3
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Molecular Weight: predicted molecular mass of 63.9 kDa after removal of the signal peptide. The apparent molecular mass of ADGRE1-His is 100-130 kDa due to glycosylation.

UniProt: Q14246