NEFL Protein (AA 9-88) (Fc Tag)

Catalog No. ABIN7092809

This Recombinant NEFL protein is expressed in HEK-293 Cells.

Quick Overview for NEFL Protein (AA 9-88) (Fc Tag) (ABIN7092809)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 9-88

Purification tag / Conjugate: This NEFL protein is labelled with Fc Tag.

Purpose: Recombinant Human NEFL(9-88) with C-terminal human Fc tag

Specificity: NEFL(Tyr9-Thr88) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NEFL

Background: CMT1F,CMT2E,CMTDIG,NF-L,NF68,NFL,PPP1R110
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Molecular Weight: predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is 35-55 kDa due to glycosylation.

UniProt: P07196