KCNK9 Protein (AA 30-79) (Fc Tag)

Catalog No. ABIN7092816

Recombinant KCNK9 protein expressed in HEK-293 Cells.

Quick Overview for KCNK9 Protein (AA 30-79) (Fc Tag) (ABIN7092816)

Target: KCNK9 (Potassium Channel, Subfamily K, Member 9 (KCNK9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-79

Purification tag / Conjugate: This KCNK9 protein is labelled with Fc Tag.

Purpose: Recombinant Human KCNK9 with C-terminal human Fc tag

Specificity: KCNK9(Glu30-Lys79) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: KCNK9 (Potassium Channel, Subfamily K, Member 9 (KCNK9))

Alternative Name: KCNK9

Background: K2p9.1,KT3.2,TASK-3,TASK3
This gene encodes a protein that contains multiple transmembrane regions and two pore-forming P domains and functions as a pH -dependent potassium channel. Amplification and overexpression of this gene have been observed in several types of human carcinomas. This gene is imprinted in the brain, with preferential expression from the maternal allele. A mutation in this gene was associated with Birk-Barel dysmorphism syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Molecular Weight: predicted molecular mass of 32.2 kDa after removal of the signal peptide. The apparent molecular mass of KCNK9-hFc is 35-55 kDa due to glycosylation.

UniProt: Q9NPC2