CD166 Protein (AA 28-526) (His tag)

Catalog No. ABIN7092822

Recombinant CD166 protein expressed in HEK-293 Cells.

Quick Overview for CD166 Protein (AA 28-526) (His tag) (ABIN7092822)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 28-526

Purification tag / Conjugate: This CD166 protein is labelled with His tag.

Purpose: Recombinant human CD166 protein with C-terminal 6xHis tag

Specificity: CD166(Trp28-Ala526) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: CD166

Background: CD166,MEMD
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Molecular Weight: predicted molecular mass of 56.8 kDa after removal of the signal peptide. The apparent molecular mass of CD166-His is 55-100 kDa due to glycosylation.

UniProt: Q13740