R-Spondin 3 Protein (RSPO3) (AA 22-146) (Fc Tag)

Catalog No. ABIN7092823

This Recombinant R-Spondin 3 protein is produced in HEK-293 Cells.

Quick Overview for R-Spondin 3 Protein (RSPO3) (AA 22-146) (Fc Tag) (ABIN7092823)

Target: R-Spondin 3 (RSPO3)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-146

Purification tag / Conjugate: This R-Spondin 3 protein is labelled with Fc Tag.

Purpose: Recombinant human RSPO3(22-146) protein with C-terminal human Fc tag

Specificity: RSPO3(Gln22-Val146) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: R-Spondin 3 (RSPO3)

Alternative Name: RSPO3

Background: CRISTIN1,PWTSR,THSD2
This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Molecular Weight: predicted molecular mass of 38.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO3(22-146)-hFc is 35-55 kDa due to glycosylation.

UniProt: Q9BXY4