CD276 Protein (CD276) (AA 29-244) (Fc Tag)

Catalog No. ABIN7092853

This Recombinant CD276 protein is produced in HEK-293 Cells.

Quick Overview for CD276 Protein (CD276) (AA 29-244) (Fc Tag) (ABIN7092853)

Target: CD276

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 29-244

Purification tag / Conjugate: This CD276 protein is labelled with Fc Tag.

Purpose: Recombinant mouse B7-H3 protein with C-terminal human Fc tag

Specificity: Mouse B7-H3(Val29-Phe244) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD276

Alternative Name: B7-H3

Background: 6030411F23Rik,AU016588,B7h3,B7RP-2
Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.[UniProtKB/Swiss-Prot Function]

Molecular Weight: predicted molecular mass of 28.7 kDa after removal of the signal peptide.The apparent molecular mass of mB7-H3-hFc is 55-70 kDa due to glycosylation.

UniProt: Q8VE98

Pathways: Cancer Immune Checkpoints