CD99 Protein (CD99) (His-Avi Tag,Biotin)
Quick Overview for CD99 Protein (CD99) (His-Avi Tag,Biotin) (ABIN7199186)
Target
See all CD99 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CD99 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human CD99 / MIC2 Protein, His,Avitag™
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Sequence
- Asp 23 - Asp 122
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Characteristics
- Biotinylated Human CD99, His,Avitag (CD9-H82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # P14209-1).
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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CD99 (CD99) (AA 23-122) protein (Fc Tag)
CD99 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD99 (CD99) (AA 23-122) protein (His tag)CD99 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD99 (CD99) (AA 25-122) protein (His-IF2DI Tag)CD99 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
CD99 (CD99) (AA 1-185) protein (GST tag)CD99 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD99 (CD99) (AA 23-122) protein (hIgG-His-tag)CD99 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
CD99 (CD99) protein (Fc Tag)CD99 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
CD99 (CD99) protein (Fc Tag)CD99 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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Application Notes
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 13.7 kDa. The protein migrates as 22 kDa and 27 kDa under reducing (R) condition due to glycosylation.
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Comment
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4
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Storage
- -20 °C
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Storage Comment
- -20°C
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- CD99
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Alternative Name
- CD99 / MIC2
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Target Type
- Viral Protein
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Background
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Synonyms: CD99,HBA71,MIC2,MIC2X,MIC2Y,MSK5X,12E7,
Description: CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A. -
Molecular Weight
- 13.7 kDa
Target
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