Albumin Protein (ALB) (His-Avi Tag,Biotin)

Catalog No. ABIN7199360

This Recombinant Albumin protein is produced in HEK-293 Cells.

Quick Overview for Albumin Protein (ALB) (His-Avi Tag,Biotin) (ABIN7199360)

Target: Albumin (ALB)

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Purification tag / Conjugate: This Albumin protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus Serum Albumin Protein, His,Avitag™ (MALS verified)

Sequence: Asp 25 - Ala 608

Characteristics: Biotinylated Cynomolgus Serum Albumin, His,Avitag (CSA-C82E5) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ala 608 (Accession # A2V9Z4-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 69.7 kDa. The protein migrates as 70 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: Synonyms: Serum albumin,ALB,Alb,
Description: serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80 % of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

Molecular Weight: 69.7 kDa

NCBI Accession: NP_001306284

Pathways: Lipid Metabolism