Actin Protein (AA 81-140) (His-IF2DI Tag)

Catalog No. ABIN7121657

This Recombinant Actin protein is produced in Escherichia coli (E. coli).

Quick Overview for Actin Protein (AA 81-140) (His-IF2DI Tag) (ABIN7121657)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 81-140

Purification tag / Conjugate: This Actin protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human ACTA1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background: ACTA, ACTA1, ACTA1 skeletal Muscle Actin, ACTA1 skeletal Muscle Actin Specific, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, sarcomeric actin

Molecular Weight: 27.7 kDa

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development