Osteopontin Protein (AA 650-920) (His-IF2DI Tag)

Catalog No. ABIN7121827

Recombinant Osteopontin protein expressed in Escherichia coli (E. coli).

Quick Overview for Osteopontin Protein (AA 650-920) (His-IF2DI Tag) (ABIN7121827)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 650-920

Purification tag / Conjugate: This Osteopontin protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human SPP1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background: AIS, ANDR_HUMAN, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease), androgen receptor splice variant 4b, AR, AR8, DHTR, Dihydro testosterone receptor

Molecular Weight: 52.1 kDa

UniProt: P10275

Pathways: Regulation of Cell Size