ATP6V1F Protein (AA 1-119) (His-IF2DI Tag)

Catalog No. ABIN7121941

Recombinant ATP6V1F protein expressed in Escherichia coli (E. coli).

Quick Overview for ATP6V1F Protein (AA 1-119) (His-IF2DI Tag) (ABIN7121941)

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-119

Purification tag / Conjugate: This ATP6V1F protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human ATP6V1F

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Alternative Name: ATP6V1F

Background: Adenosinetriphosphatase 14k chain, ATP6S14, ATP6V1F, ATPase, H+ transporting, lysosomal 14 kDa, V1 subunit F, ATPase, vacuolar, 14 kD, H(+)-transporting two-sector ATPase, 14kD subunit, MGC117321, MGC126037, MGC126038, V-ATPase 14 kDa subunit, V-ATPase subunit F

Molecular Weight: 13 kDa

UniProt: Q16864

Pathways: Transition Metal Ion Homeostasis, Proton Transport