CCL8 Protein (AA 24-99) (His tag)

Catalog No. ABIN7122314

This Recombinant CCL8 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL8 Protein (AA 24-99) (His tag) (ABIN7122314)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-99

Purification tag / Conjugate: This CCL8 protein is labelled with His tag.

Purpose: Recombinant Human CCL8

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 0.1-0.5 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8

Background: Ccl8, CCL8_HUMAN, HC14, MCP-2, MCP-2(6-76), Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, SCYA10, SCYA8, Small-inducible cytokine A8

Molecular Weight: 10.8 kDa

UniProt: P80075