GLO1 Protein (AA 2-184) (His tag)

Catalog No. ABIN7123173

This Recombinant GLO1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GLO1 Protein (AA 2-184) (His tag) (ABIN7123173)

Target: GLO1 (Glyoxalase I (GLO1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 2-184

Purification tag / Conjugate: This GLO1 protein is labelled with His tag.

Purpose: Recombinant Human GLO1

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: GLO1

Background: Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase

Molecular Weight: 22.5 kDa

UniProt: Q04760