Histidine--tRNA Ligase (HARS-1) (AA 1-509) protein (His tag)

Catalog No. ABIN7123338

This Recombinant Histidine--tRNA Ligase protein is expressed in Escherichia coli (E. coli).

Quick Overview for Histidine--tRNA Ligase (HARS-1) (AA 1-509) protein (His tag) (ABIN7123338)

Target: Histidine--tRNA Ligase (HARS-1)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-509

Purification tag / Conjugate: His tag

Purpose: Recombinant Human HARS1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Histidine--tRNA Ligase (HARS-1)

Alternative Name: HARS1

Background: Cytoplasmic, EC 6.1.1.21, FLJ20491, HARS, HisRS, Histidine tRNA ligase, cytoplasmic, histidine translase, Histidine tRNA ligase, Histidine--tRNA ligase, Histidyl tRNA synthetase, Histidyl-tRNA synthetase, HRS, Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds, SYHC_HUMAN, USH3B

Molecular Weight: 59.3 kDa

UniProt: P12081