Interferon gamma Protein (IFNG) (AA 24-166)
Quick Overview for Interferon gamma Protein (IFNG) (AA 24-166) (ABIN7123486)
Target
See all Interferon gamma (IFNG) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 24-166
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Purpose
- Recombinant Human IFNG
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 0.01 EU/μg as determined by LAL test.
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Biological Activity Comment
- Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-0.7 ng/mL.
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Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag)
IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag,Biotin)IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (Active) proteinIFNG Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Origin: Hamster Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 23-155) (Active) protein (His tag)IFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE. WB, ELISA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 5 % Sucrose, 4 % Mannitol, 0.02 % Tween 80, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- Interferon gamma (IFNG)
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Alternative Name
- IFNG
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Background
- Interferon gamma, IFN-gamma, Immune interferon
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Molecular Weight
- 16 kDa
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UniProt
- P01579
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Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Target
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