ME1 Protein (AA 226-572) (His tag)
Quick Overview for ME1 Protein (AA 226-572) (His tag) (ABIN7123839)
Target
See all ME1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 226-572
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Purification tag / Conjugate
- This ME1 protein is labelled with His tag.
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Purpose
- Recombinant Human ME1
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Sterility
- 0.2 μm filtered
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Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)
custom-made ME1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)custom-made ME1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (GST tag)ME1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) protein (Myc-DYKDDDDK Tag)ME1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
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Alternative Name
- ME1
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Background
- HUMNDME, Malic enzyme 1, ME1, MES, NADP dependent malic enzyme, NADP ME
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Molecular Weight
- 40 kDa
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UniProt
- P48163
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Pathways
- Regulation of Lipid Metabolism by PPARalpha
Target
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