NCK1 Protein (AA 1-377) (His tag)

Catalog No. ABIN7124117

This Recombinant NCK1 protein is produced in Escherichia coli (E. coli).

Quick Overview for NCK1 Protein (AA 1-377) (His tag) (ABIN7124117)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE

Product Details

Protein Characteristics: AA 1-377

Purification tag / Conjugate: This NCK1 protein is labelled with His tag.

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: Nck

Background: Cytoplasmic protein NCK1, Melanoma Nck protein, MGC12668, NCK, NCK 1, NCK adaptor protein 1, NCK alpha, NCK tyrosine kinase, Nck-1, NCK1, NCK1_HUMAN, NCKalpha, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK-alpha

Molecular Weight: 41.4 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals