GADD34 Protein (AA 464-674) (His tag)

Catalog No. ABIN7124666

This Recombinant GADD34 protein is produced in Escherichia coli (E. coli).

Quick Overview for GADD34 Protein (AA 464-674) (His tag) (ABIN7124666)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 464-674

Purification tag / Conjugate: This GADD34 protein is labelled with His tag.

Purpose: Recombinant Human PPP1R15A

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: PPP1R15A

Background: Apoptosis associated protein, GADD 34, Growth arrest and DNA damage inducible 34, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, Ppp1r15a, PR15A_HUMAN, Protein phosphatase 1 regulatory (inhibitor) subunit 15A

Molecular Weight: 25.13 kDa

UniProt: O75807

Pathways: ER-Nucleus Signaling, Unfolded Protein Response